DNA

Part:BBa_K1732008:Design

Designed by: Donna Lee   Group: iGEM15_Carnegie_Mellon   (2015-09-09)


pSB1C3-J23100-B0034-EGFP-His-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 212
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 77


Design Notes

Sequence is codon optimized for E.coli


Source

Sequence designed by Cheryl Telmer from Bruchez lab in Carnegie Mellon University.

References